Complexin 4 antibody

Catalog No. ABIN1742267

Product Details anti-Complexin 4 Antibody

Target: Complexin 4 (CPLX4)

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complexin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for complexin 4

Cross-Reactivity (Details): no cross reaction to other complexins.

Purification: purified IgG

Immunogen: Recombinant full length protein of mouse complexin 4.

Clone: 171H8

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 (ECL detection)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 2000
IHC-P: 1 : 200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Complexin 4 antibody (ABIN1742267)

Vaithianathan, Henry, Akmentin, Matthews: "Functional roles of complexin in neurotransmitter release at ribbon synapses of mouse retinal bipolar neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 9, pp. 4065-70, (2015) (PubMed).

Landgraf, Mühlhans, Dedek, Reim, Brandstätter, Ammermüller: "The absence of Complexin 3 and Complexin 4 differentially impacts the ON and OFF pathways in mouse retina." in: The European journal of neuroscience, Vol. 36, Issue 4, pp. 2470-81, (2012) (PubMed).

Xue, Stradomska, Chen, Brose, Zhang, Rosenmund, Reim: "Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 22, pp. 7875-80, (2008) (PubMed).

Target Details for Complexin 4

Target: Complexin 4 (CPLX4)

Alternative Name: Complexin 4 (CPLX4 Products)

Synonyms: CPLX4 antibody, zgc:153429 antibody, si:ch211-233a1.5 antibody, CPX-IV antibody, CPXIV antibody, A930004D23Rik antibody, Gm957 antibody, complexin 4 antibody, complexin 4a antibody, CPLX4 antibody, cplx4a antibody, Cplx4 antibody

Background: Synonyms: CPX 4