Amphiphysin antibody (AA 2-15)

Catalog No. ABIN1742262

Product Details anti-Amphiphysin Antibody

Target: Amphiphysin (AMPH)

Binding Specificity: AA 2-15

Reactivity: Human, Mouse, Rat, Chicken, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amphiphysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Specific for amphiphysin.

Purification: antiserum

Immunogen: Synthetic peptide ADIKTGIFAKNVQK (aa 2-15 of amphiphysin I) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Amphiphysin antibody (ABIN1742262)

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Neef, Jung, Wong, Reuter, Pangrsic, Chakrabarti, Kügler, Lenz, Nouvian, Boumil, Frankel, Wichmann, Moser: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

von Kleist, Stahlschmidt, Bulut, Gromova, Puchkov, Robertson, MacGregor, Tomilin, Tomlin, Pechstein, Chau, Chircop, Sakoff, von Kries, Saenger, Kräusslich, Shupliakov, Robinson, McCluskey, Haucke: "Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition." in: Cell, Vol. 146, Issue 3, pp. 471-84, (2011) (PubMed).

Scott, Tabarean, Tang, Cartier, Masliah, Roy: "A pathologic cascade leading to synaptic dysfunction in alpha-synuclein-induced neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8083-95, (2010) (PubMed).

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Basheer, Brown, Ramesh, Begum, McCarley: "Sleep deprivation-induced protein changes in basal forebrain: implications for synaptic plasticity." in: Journal of neuroscience research, Vol. 82, Issue 5, pp. 650-8, (2005) (PubMed).

Fernández-Chacón, Achiriloaie, Janz, Albanesi, Südhof: "SCAMP1 function in endocytosis." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12752-6, (2000) (PubMed).

Von Kriegstein, Schmitz, Link, Südhof: "Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses." in: The European journal of neuroscience, Vol. 11, Issue 4, pp. 1335-48, (1999) (PubMed).

Target Details for Amphiphysin

Target: Amphiphysin (AMPH)

Alternative Name: Amphiphysin (AMPH Products)