SV2B antibody (AA 2-17)

Catalog No. ABIN1742258

Product Details anti-SV2B Antibody

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Binding Specificity: AA 2-17

Reactivity: Human, Mouse, Rat, Hamster, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SV2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for SV2 B.

Purification: antiserum

Immunogen: Synthetic peptide DDYRYRDNYEGYAPND (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 200

Comment:

WB: SV2 aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SV2B antibody (ABIN1742258)

Fukusumi, Wakamatsu, Takashima, Hasegawa, Miyauchi, Tomita, Kawachi: "SV2B is essential for the integrity of the glomerular filtration barrier." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 95, Issue 5, pp. 534-45, (2015) (PubMed).

Crèvecoeur, Kaminski, Rogister, Foerch, Vandenplas, Neveux, Mazzuferi, Kroonen, Poulet, Martin, Sadzot, Rikir, Klitgaard, Moonen, Deprez: "Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis." in: Neuropathology and applied neurobiology, Vol. 40, Issue 2, pp. 191-204, (2014) (PubMed).

Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

Mahrhold, Strotmeier, Garcia-Rodriguez, Lou, Marks, Rummel, Binz: "Identification of the SV2 protein receptor-binding site of botulinum neurotoxin type E." in: The Biochemical journal, Vol. 453, Issue 1, pp. 37-47, (2013) (PubMed).

Crèvecœur, Foerch, Doupagne, Thielen, Vandenplas, Moonen, Deprez, Rogister: "Expression of SV2 isoforms during rodent brain development." in: BMC neuroscience, Vol. 14, pp. 87, (2013) (PubMed).

Couesnon, Molgó, Connan, Popoff: "Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine." in: PLoS pathogens, Vol. 8, Issue 3, pp. e1002583, (2012) (PubMed).

Lazarevic, Schöne, Heine, Gundelfinger, Fejtova: "Extensive remodeling of the presynaptic cytomatrix upon homeostatic adaptation to network activity silencing." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 28, pp. 10189-200, (2011) (PubMed).

Boido, Farisello, Cesca, Ferrea, Valtorta, Benfenati, Baldelli: "Cortico-hippocampal hyperexcitability in synapsin I/II/III knockout mice: age-dependency and response to the antiepileptic drug levetiracetam." in: Neuroscience, Vol. 171, Issue 1, pp. 268-83, (2010) (PubMed).

Yeh, Dong, Yao, Tepp, Lin, Johnson, Chapman: "SV2 mediates entry of tetanus neurotoxin into central neurons." in: PLoS pathogens, Vol. 6, Issue 11, pp. e1001207, (2010) (PubMed).

Morgans, Kensel-Hammes, Hurley, Burton, Idzerda, McKnight, Bajjalieh: "Loss of the Synaptic Vesicle Protein SV2B results in reduced neurotransmission and altered synaptic vesicle protein expression in the retina." in: PLoS ONE, Vol. 4, Issue 4, pp. e5230, (2009) (PubMed).

Couesnon, Pereira, Popoff: "Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers." in: Cellular microbiology, Vol. 10, Issue 2, pp. 375-87, (2008) (PubMed).

Verderio, Grumelli, Raiteri, Coco, Paluzzi, Caccin, Rossetto, Bonanno, Montecucco, Matteoli: "Traffic of botulinum toxins A and E in excitatory and inhibitory neurons." in: Traffic (Copenhagen, Denmark), Vol. 8, Issue 2, pp. 142-53, (2007) (PubMed).

Miyauchi, Saito, Karasawa, Harita, Suzuki, Koike, Han, Shimizu, Kawachi: "Synaptic vesicle protein 2B is expressed in podocyte, and its expression is altered in proteinuric glomeruli." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 10, pp. 2748-59, (2006) (PubMed).

Schivell, Mochida, Kensel-Hammes, Custer, Bajjalieh: "SV2A and SV2C contain a unique synaptotagmin-binding site." in: Molecular and cellular neurosciences, Vol. 29, Issue 1, pp. 56-64, (2005) (PubMed).

von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

Redecker, Kreutz, Bockmann, Gundelfinger, Boeckers: "Brain synaptic junctional proteins at the acrosome of rat testicular germ cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 51, Issue 6, pp. 809-19, (2003) (PubMed).

Janz, Südhof: "SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family." in: Neuroscience, Vol. 94, Issue 4, pp. 1279-90, (2000) (PubMed).

Target Details for SV2B

Target: SV2B (Synaptic Vesicle Glycoprotein 2B (SV2B))

Alternative Name: SV2 B (SV2B Products)

Synonyms: SV2B antibody, A830038F04Rik antibody, AI850768 antibody, mKIAA0735 antibody, HsT19680 antibody, synaptic vesicle glycoprotein 2B antibody, synaptic vesicle glycoprotein 2 b antibody, synaptic vesicle glycoprotein 2b antibody, SV2B antibody, CpipJ_CPIJ009611 antibody, Sv2b antibody