For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in Tris buffer. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handling Advice
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage
-20 °C
Storage Comment
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Yang, Farias, Kapfhamer, Tobias, Grant, Abel, Bućan: "Biochemical, molecular and behavioral phenotypes of Rab3A mutations in the mouse." in: Genes, brain, and behavior, Vol. 6, Issue 1, pp. 77-96, (2007) (PubMed).