WB: 1 : 1000 up to 1 : 5000 (AP staining) ICC: 1 : 500 up to 1 : 1000 IHC: 1 : 1000 up to 1 : 5000
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS
Handling Advice
Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C. Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Storage
4 °C/-20 °C
Storage Comment
Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Neef, Jung, Wong, Reuter, Pangrsic, Chakrabarti, Kügler, Lenz, Nouvian, Boumil, Frankel, Wichmann, Moser: "Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 3, pp. 705-16, (2014) (PubMed).
Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).