Dynamin 1, 2, 3 (AA 2-17) antibody
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- Target
- Dynamin 1, 2, 3
- Binding Specificity
- AA 2-17
- Reactivity
- Cow, Human, Mouse, Rat
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Recognizes dynamin 1, 2 and 3 with a preference for dynamin 1.
- Purification
- antiserum
- Immunogen
- Synthetic peptide GNRGMEDLIPLVNRLQ (aa 2-17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
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- Application Notes
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WB: 1 : 1000 (AP staining)
ICC: 1 : 200 up to 1 : 1000
IHC-P: 1 : 100 - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS
- Handling Advice
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. - Storage
- 4 °C/-20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "Increased neurotransmitter release at the neuromuscular junction in a mouse model of polyglutamine disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 3, pp. 1106-13, (2011) (PubMed).
: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).
: "Dynamic interaction of amphiphysin with N-WASP regulates actin assembly." in: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 34244-56, (2009) (PubMed).
: "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis." in: Science (New York, N.Y.), Vol. 316, Issue 5824, pp. 570-4, (2007) (PubMed).
: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).
: "
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Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).
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- Target
- Dynamin 1, 2, 3
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