NMDA 1 Receptor antibody (AA 35-53)

Catalog No. ABIN1742247

Product Details anti-NMDA 1 Receptor Antibody

Target: NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))

Binding Specificity: AA 35-53

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMDA 1 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for GluN 1.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 35-53 of rat GluN 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: not recommended
IP: not tested yet
ICC: not recommended
IHC: 1 : 500 up to 1 : 1000

Comment:

WB: Antibody 1, ABIN1742246, is recommended for WB. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008). Cell-type-dependent molecular composition of the axon initial segment. Journal of Neuroscience 28: 14329-40.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-NMDA 1 Receptor antibody (ABIN1742247)

Prüss, Leubner, Wenke, Czirják, Szentiks, Greenwood: "Anti-NMDA Receptor Encephalitis in the Polar Bear (Ursus maritimus) Knut." in: Scientific reports, Vol. 5, pp. 12805, (2015) (PubMed).

Target Details for NMDA 1 Receptor

Target: NMDA 1 Receptor (NMDA R1) (NMDA Receptor 1 (NMDA R1))

Alternative Name: NMDA-Receptor 1 (NMDA R1 Products)

Synonyms: CG2902 antibody, DMNMDAR1 antibody, DNMDAR-I antibody, DNmdar antibody, DmelNmdar1 antibody, Dmel\\CG2902 antibody, DrNR1 antibody, NMDA antibody, NMDA R1 antibody, NMDAR1 antibody, NMdar1 antibody, NR1 antibody, NRI antibody, Nmdar antibody, dNR1 antibody, dnmdar-I antibody, nmr antibody, NMDA receptor 1 antibody, Nmdar1 antibody