SNAP alpha/beta antibody
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- Target See all SNAP alpha/beta products
- SNAP alpha/beta
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Reactivity
- Human, Rat, Zebrafish (Danio rerio), Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SNAP alpha/beta antibody is un-conjugated
- Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Specificity
- Specific for alpha- and beta SNAP
- Cross-Reactivity (Details)
- does not cross-react to gamma SNAP.
- Purification
- purified IgG
- Immunogen
- Recombinant full length rat alpha SNAP.
- Clone
- 77-2
- Isotype
- IgG1
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- Application Notes
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WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: not tested yet - Comment
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The antibody does not immunoprecipitate the 20 S SNARE-complex.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Storage
- -20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).
: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).
: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).
: "
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Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).
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- Target
- SNAP alpha/beta
- Abstract
- SNAP alpha/beta Products
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