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SNAP alpha/beta antibody

Reactivity: Human, Rat, Zebrafish (Danio rerio), Mouse WB, ICC, IP Host: Mouse Monoclonal 77-2 unconjugated
Catalog No. ABIN1742242
  • Target See all SNAP alpha/beta products
    SNAP alpha/beta
    Reactivity
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Zebrafish (Danio rerio), Mouse
    Host
    • 2
    • 1
    Mouse
    Clonality
    • 2
    • 1
    Monoclonal
    Conjugate
    • 3
    This SNAP alpha/beta antibody is un-conjugated
    Application
    Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    Specificity
    Specific for alpha- and beta SNAP
    Cross-Reactivity (Details)
    does not cross-react to gamma SNAP.
    Purification
    purified IgG
    Immunogen
    Recombinant full length rat alpha SNAP.
    Clone
    77-2
    Isotype
    IgG1
  • Application Notes
    WB: 1 : 1000 up to 1 : 10000 (AP staining)
    ICC: 1 : 500 up to 1 : 1000
    IHC: not tested yet
    Comment

    The antibody does not immunoprecipitate the 20 S SNARE-complex.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Handling Advice
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    Storage
    -20 °C
    Storage Comment
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

  • Target
    SNAP alpha/beta
    Abstract
    SNAP alpha/beta Products
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