SNAP alpha/beta antibody

Catalog No. ABIN1742241

Product Details anti-SNAP alpha/beta Antibody

Target: SNAP alpha/beta

Reactivity: Human, Rat, Zebrafish (Danio rerio), Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNAP alpha/beta antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for alpha- and beta SNAP

Cross-Reactivity (Details): does not cross-react to gamma SNAP.

Purification: purified IgG

Immunogen: Recombinant full length rat alpha SNAP

Clone: 77-1

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: no (denaturation of the antigen during fixation)
IHC: not tested yet

Comment:

The antibody does not immunoprecipitate the 20 S SNARE-complex.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-SNAP alpha/beta antibody (ABIN1742241)

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, (2010) (PubMed).

Hanson, Otto, Barton, Jahn: "The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16955-61, (1995) (PubMed).

Target Details for SNAP alpha/beta

Target: SNAP alpha/beta

Abstract: SNAP alpha/beta Products