SNAP47 antibody

Catalog No. ABIN1742240

Product Details anti-SNAP47 Antibody

Target: SNAP47 (Synaptosomal-Associated Protein, 47kDa (SNAP47))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP47 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Specificity: Specific for SNAP 47.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant full length rat SNAP 47.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-SNAP47 antibody (ABIN1742240)

Biesemann, Grønborg, Luquet, Wichert, Bernard, Bungers, Cooper, Varoqueaux, Li, Byrne, Urlaub, Jahn, Brose, Herzog: "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting." in: The EMBO journal, Vol. 33, Issue 2, pp. 157-70, (2014) (PubMed).

Walter, Kurps, de Wit, Schöning, Toft-Bertelsen, Lauks, Ziomkiewicz, Weiss, Schulz, Fischer von Mollard, Verhage, Sørensen: "The SNARE protein vti1a functions in dense-core vesicle biogenesis." in: The EMBO journal, Vol. 33, Issue 15, pp. 1681-97, (2014) (PubMed).

Holt, Varoqueaux, Wiederhold, Takamori, Urlaub, Fasshauer, Jahn: "Identification of SNAP-47, a novel Qbc-SNARE with ubiquitous expression." in: The Journal of biological chemistry, Vol. 281, Issue 25, pp. 17076-83, (2006) (PubMed).

Target Details for SNAP47

Target: SNAP47 (Synaptosomal-Associated Protein, 47kDa (SNAP47))

Alternative Name: SNAP 47 (SNAP47 Products)

Synonyms: 1110031B06Rik antibody, SNAP-47 antibody, RGD735194 antibody, zgc:153222 antibody, C1orf142 antibody, HEL170 antibody, SVAP1 antibody, C7H1orf142 antibody, synaptosome associated protein 47 antibody, synaptosome associated protein 47kDa L homeolog antibody, synaptosome associated protein 47kDa antibody, synaptosomal-associated protein, 47 antibody, SNAP47 antibody, snap47.L antibody, snap47 antibody, Snap47 antibody

Background: Synonyms: SVAP 1