WB: 1 : 1000 (AP staining) IP: not tested yet ICC: 1 : 100 IHC: not tested yet
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handling Advice
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage
-20 °C
Storage Comment
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Varadarajan, Bampton, Smalley, Tanaka, Caves, Butterworth, Wei, Pellecchia, Mitcheson, Gant, Dinsdale, Cohen: "A novel cellular stress response characterised by a rapid reorganisation of membranes of the endoplasmic reticulum." in: Cell death and differentiation, Vol. 19, Issue 12, pp. 1896-907, (2012) (PubMed).