STX8 antibody (Cytoplasmic Domain)

Catalog No. ABIN1742226

Product Details anti-STX8 Antibody

Target: STX8 (Syntaxin 8 (STX8))

Binding Specificity: AA 1-215, Cytoplasmic Domain

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX8 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for syntaxin 8.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant cytoplasmic domain of rat syntaxin 8 (aa 1-215).

Application Details

Application Notes: WB: 1 : 100 up to 1 : 5000 (AP staining)
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-STX8 antibody (ABIN1742226)

Pattu, Qu, Schwarz, Strauss, Weins, Bhat, Halimani, Marshall, Rettig, Hoth: "SNARE protein expression and localization in human cytotoxic T lymphocytes." in: European journal of immunology, Vol. 42, Issue 2, pp. 470-5, (2012) (PubMed).

Madan, Rastogi, Parashuraman, Mukhopadhyay: "Salmonella acquires lysosome-associated membrane protein 1 (LAMP1) on phagosomes from Golgi via SipC protein-mediated recruitment of host Syntaxin6." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5574-87, (2012) (PubMed).

Offenhäuser, Lei, Roy, Collins, Stow, Murray: "Syntaxin 11 binds Vti1b and regulates late endosome to lysosome fusion in macrophages." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 6, pp. 762-73, (2011) (PubMed).

Flowerdew, Burgoyne: "A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels." in: The Biochemical journal, Vol. 418, Issue 3, pp. 529-40, (2009) (PubMed).

Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).

Murray, Wylie, Khromykh, Hume, Stow: "Syntaxin 6 and Vti1b form a novel SNARE complex, which is up-regulated in activated macrophages to facilitate exocytosis of tumor necrosis Factor-alpha." in: The Journal of biological chemistry, Vol. 280, Issue 11, pp. 10478-83, (2005) (PubMed).

Nakamura, Fukuda, Kato, Hirose: "MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 1696-710, (2005) (PubMed).

Target Details for STX8

Target: STX8 (Syntaxin 8 (STX8))

Alternative Name: Syntaxin 8 (STX8 Products)

Pathways: Synaptic Vesicle Exocytosis