Syntaxin 1B antibody (AA 171-187)

Catalog No. ABIN1742218

Product Details anti-Syntaxin 1B Antibody

Target: Syntaxin 1B (STX1B)

Binding Specificity: AA 171-187

Reactivity: Human, Mouse, Chicken, Cow, Hamster, Rat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 1B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Specific for syntaxin 1B

Cross-Reactivity (Details): no cross reactivity to syntaxin 1A.

Purification: antiserum

Immunogen: Synthetic peptide GKLAIFTDDIKMDSQMT (aa 171-187 in rat syntaxin 1B) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 1000
IHC: 1 : 1000
IHC-P: 1 : 500
ELISA: not tested yet

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Syntaxin 1B antibody (ABIN1742218)

Zhou, Liu, Yu, Han, Fan, Shao, Chen, Qiao, Xie: "Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats." in: The international journal of neuropsychopharmacology, Vol. 19, Issue 1, (2016) (PubMed).

Arsenault, Ferrari, Niranjan, Cuijpers, Gu, Vallis, OBrien, Davletov: "Stapling of the botulinum type A protease to growth factors and neuropeptides allows selective targeting of neuroendocrine cells." in: Journal of neurochemistry, Vol. 126, Issue 2, pp. 223-33, (2013) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Kabayama, Takeuchi, Taniguchi, Tokushige, Kozaki, Mizutani, Nakamura, Mikoshiba: "Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 20, pp. 7357-64, (2011) (PubMed).

Tsai, Garcia-Gil, van Haeften, Gadella: "How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane." in: PLoS ONE, Vol. 5, Issue 6, pp. e11204, (2010) (PubMed).

Sander, Frank, Bolat, Blank, Galli, Bigalke, Bischoff, Lorentz: "Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells." in: European journal of immunology, Vol. 38, Issue 3, pp. 855-63, (2008) (PubMed).

Kubota, Kobayashi, Mori, Higashide, McLaren, Inana: "Changes in retinal synaptic proteins in the transgenic model expressing a mutant HRG4 (UNC119)." in: Investigative ophthalmology & visual science, Vol. 43, Issue 2, pp. 308-13, (2002) (PubMed).

Low, Marmorstein, Miura, Li, Kudo, Marmorstein, Weimbs: "Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype." in: Journal of cell science, Vol. 115, Issue Pt 23, pp. 4545-53, (2002) (PubMed).

Target Details for Syntaxin 1B

Target: Syntaxin 1B (STX1B)

Alternative Name: Syntaxin 1B (STX1B Products)

Background: Synonyms: p35B