VAMP2 antibody (AA 2-17)

Catalog No. ABIN1742194

Product Details anti-VAMP2 Antibody

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Binding Specificity: AA 2-17

Reactivity: Human, Mouse, Rat, Hamster

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This VAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for VAMP 2

Cross-Reactivity (Details): no cross reactivity to VAMP 1 and VAMP 3

Purification: antiserum

Immunogen: Synthetic peptide SATAATVPPAAPAGEG (aa 2-17 in rat synaptobrevin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 1000
IHC: 1 : 500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-VAMP2 antibody (ABIN1742194)

van Stegen, Dagar, Gottmann: "Release activity-dependent control of vesicle endocytosis by the synaptic adhesion molecule N-cadherin." in: Scientific reports, Vol. 7, pp. 40865, (2017) (PubMed).

Schaefer, Toral, Velez, Cox, Baker, Borcherding, Colgan, Bondada, Mashburn, Yu, Geddes, Tsang, Bassuk, Mahajan: "Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2509-21, (2016) (PubMed).

Kung, Gong, Adedoyin, Ng, Bhargava, Ohara, Jasmin: "Evidence for glutamate as a neuroglial transmitter within sensory ganglia." in: PLoS ONE, Vol. 8, Issue 7, pp. e68312, (2013) (PubMed).

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: Synaptobrevin 2 (VAMP2 Products)

Synonyms: zgc:73302 antibody, wu:fj61c06 antibody, VAMP2 antibody, vamp2-a antibody, xsybi antibody, Syb-2 antibody, Syb2 antibody, sybII antibody, RATVAMPB antibody, RATVAMPIR antibody, SYB antibody, SYB2 antibody, VAMP-2 antibody, syb2 antibody, vamp2 antibody, xsybii antibody, vesicle-associated membrane protein 2 antibody, vesicle associated membrane protein 2 antibody, vesicle associated membrane protein 2 S homeolog antibody, vesicle-associated membrane protein 2 (synaptobrevin 2) antibody, vesicle associated membrane protein 2 L homeolog antibody, vamp2 antibody, VAMP2 antibody, vamp2.S antibody, Vamp2 antibody, vamp2.L antibody

Background: Synonyms: VAMP 2, p18-2

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport