Synaptogyrin 1 antibody (AA 170-234)

Catalog No. ABIN1742188

Product Details anti-Synaptogyrin 1 Antibody

Target: Synaptogyrin 1 (SYNGR1)

Binding Specificity: AA 170-234

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Synaptogyrin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for synaptogyrin 1 with a weak side-reactivity towards tubulin.

Purification: purified IgG

Immunogen: Recombinant rat synaptogyrin 1 (aa 170-234).

Clone: 80-1

Isotype: IgG1

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: 1 : 100

Comment:

IP: In immunoprecipitation (but not in other assays) tubulin coprecipitates with synaptogyrin 1 in about equal proportions. This is due to a direct binding of the antibody to tubulin. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742189 as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References for anti-Synaptogyrin 1 antibody (ABIN1742188)

von Kriegstein, Schmitz: "The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina." in: Cell and tissue research, Vol. 311, Issue 2, pp. 159-73, (2003) (PubMed).

Stenius, Janz, Südhof, Jahn: "Structure of synaptogyrin (p29) defines novel synaptic vesicle protein." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 2, pp. 1801-9, (1996) (PubMed).

Target Details for Synaptogyrin 1

Target: Synaptogyrin 1 (SYNGR1)

Alternative Name: Synaptogyrin 1 (SYNGR1 Products)

Background: Synonyms: p29

Pathways: Regulation of long-term Neuronal Synaptic Plasticity