pADPR antibody (Biotin)
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- Target
- pADPR
- Reactivity
- All Species
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Biotin
- Application
- Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)
- Characteristics
- Recognizes DNA-damage and apoptosis related poly-ADP ribosylated proteins and poly (ADP-ribose) polymer
- Purification
- Protein A affinity column
- Immunogen
- poly(ADP-ribose)
- Clone
- 10H
- Isotype
- IgG3
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- Application Notes
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WB 5 µg/mL,
IH 2 µg/mL - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- PBS, pH 7.4 with 0,05 % sodium azides
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Stable for 1 year from date of shipment when stored at -20 °C or -70 °C. Stable for at least 1 month at 4 °C.
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Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains." in: Structure (London, England : 1993), Vol. 21, Issue 3, pp. 462-75, (2013) (PubMed).
: "CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12975-84, (2012) (PubMed).
: "Tankyrase-1 polymerization of poly(ADP-ribose) is required for spindle structure and function." in: Nature cell biology, Vol. 7, Issue 11, pp. 1133-9, (2005) (PubMed).
: "
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Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains." in: Structure (London, England : 1993), Vol. 21, Issue 3, pp. 462-75, (2013) (PubMed).
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- Target
- pADPR
- Background
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Synonyms: poly(ADP-ribose)
Poly(ADP-ribose) or pADPr is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerase (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer pADPr, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. pADPr becomes attached to nuclear proteins, and to PARP itself (automodification).
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