KCNK3 antibody (AA 251-411)

Catalog No. ABIN1741485

Product Details anti-KCNK3 Antibody

Target: KCNK3 (Potassium Channel, Subfamily K, Member 3 (KCNK3))

Binding Specificity: AA 251-411

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~50 kDa. Does not cross-react with TASK3.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 251-411 (cytoplasmic C-terminus) of rat Acid-sensitive potassium channel protein TASK or TASK1. Mouse: 96% identity (156/161 amino acids identical). Human: 76% identity (163/161 amino acids identical). <30% identity with TASK3.

Clone: S374-48

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1741485 was sufficient for detection of TASK1 Potassium Channel in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for KCNK3

Target: KCNK3 (Potassium Channel, Subfamily K, Member 3 (KCNK3))

Alternative Name: KCNK3 (KCNK3 Products)

Synonyms: si:dkey-164m15.1 antibody, K2p3.1 antibody, OAT1 antibody, PPH4 antibody, TASK antibody, TASK-1 antibody, TBAK1 antibody, cTBAK-1 antibody, Task-1 antibody, rTASK antibody, KCNK3 antibody, potassium channel, subfamily K, member 3a antibody, potassium two pore domain channel subfamily K member 3 antibody, potassium channel, subfamily K, member 3 antibody, kcnk3a antibody, KCNK3 antibody, Kcnk3 antibody

Background: K+ channels are divided into three subclasses reflecting the number of transmembrane segments (TMS), which are designated 6TMS, 4TMS and 2TMS. Members of the 4TMS class contain two distinct pore regions and include TWIK, TREK, TRAAK and TASK. TASK channels are highly sensitive to external pH in the physiological range. TASK-1 is expressed in brain and in rat heart, with high levels of expression in the right atrium. TASK-2, mainly expressed in kidney, is localized in cortical distal tubules and collecting ducts, suggesting a role in renal K+ transport. TASK-3 from rat cerebellum shares 54 % identity with TASK-1, but less than 30 % identity with TASK-2 and other tandem pore K+ channels.

Gene ID: 29553

NCBI Accession: NP_203694

UniProt: O54912