Neuroligin 3 antibody (AA 730-848) (HRP)

Catalog No. ABIN1741462

Product Details anti-Neuroligin 3 Antibody (HRP)

Target: Neuroligin 3 (NLGN3)

Binding Specificity: AA 730-848

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neuroligin 3 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~110 kDa. Does not cross-react with Neuroligin-1, -2, -4 or -4.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3. Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.

Clone: S110-29

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1741462 was sufficient for detection of Neuroligin 3 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Neuroligin 3

Target: Neuroligin 3 (NLGN3)

Alternative Name: Neuroligin 3 (NLGN3 Products)

Synonyms: Nlgn3 antibody, GB18290 antibody, hnl3 antibody, NLGN3 antibody, CCE-L1 antibody, NV16744 antibody, Nv1604741 antibody, HNL3 antibody, A230085M13Rik antibody, NL3 antibody, neuroligin 3 antibody, neuroligin 3 S homeolog antibody, NLG-3 antibody, nlgn3.S antibody, NLGN3 antibody, LOC659852 antibody, LOC100121155 antibody, nlgn3 antibody, Nlgn3 antibody

Background: Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses.

Gene ID: 171297

UniProt: Q62889

Pathways: Synaptic Membrane