LGI1 antibody (AA 37-113)

Catalog No. ABIN1741269

Product Details anti-LGI1 Antibody

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Binding Specificity: AA 37-113

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~60 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.

Clone: S283-7

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1741269 was sufficient for detection of LGI1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for LGI1

Target: LGI1 (Leucine-Rich, Glioma Inactivated 1 (LGI1))

Alternative Name: LGI1 (LGI1 Products)

Synonyms: ADLTE antibody, ADPAEF antibody, ADPEAF antibody, EPITEMPIN antibody, EPT antibody, ETL1 antibody, IB1099 antibody, BB130740 antibody, MGC146600 antibody, LGIa antibody, fb78h12 antibody, wu:fb78h12 antibody, zgc:65886 antibody, leucine rich glioma inactivated 1 antibody, leucine-rich repeat LGI family, member 1 antibody, leucine-rich, glioma inactivated 1 antibody, leucine-rich, glioma inactivated 1b antibody, leucine-rich, glioma inactivated 1a antibody, LGI1 antibody, Lgi1 antibody, lgi1 antibody, lgi1b antibody, lgi1a antibody

Background: The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist, this LGI1 antibody will recognize only the longer form.

Gene ID: 56839

NCBI Accession: NP_064674

UniProt: Q9JIA1