PINK1 antibody (AA 112-496)

Catalog No. ABIN1741107

Product Details anti-PINK1 Antibody

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Binding Specificity: AA 112-496

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PINK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~50 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1. 82% identical to rat and 81% identical to mouse. >30% identity with DMPK.

Clone: S4-15

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1741107 was sufficient for detection of PINK1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for PINK1

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Alternative Name: PINK1 (PINK1 Products)

Synonyms: BEST:GH23468 antibody, CG4523 antibody, Dmel\\CG4523 antibody, PINK antibody, PINK1 antibody, dPINK1 antibody, dPink1 antibody, pink1 antibody, wu:fc39e12 antibody, zgc:101729 antibody, BRPK antibody, PARK6 antibody, 1190006F07Rik antibody, AU042772 antibody, AW557854 antibody, mFLJ00387 antibody, PTEN-induced putative kinase 1 antibody, PTEN induced putative kinase 1 antibody, Pink1 antibody, PINK1 antibody, pink1 antibody

Background: PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kD protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kD and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports, PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction.

Gene ID: 65018

NCBI Accession: NP_115785

UniProt: Q9BXM7

Pathways: Autophagy