ATF4 antibody (AA 25-327) (HRP)
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- Target See all ATF4 Antibodies
- ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))
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Binding Specificity
- AA 25-327
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ATF4 antibody is conjugated to HRP
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Detects ~60 kDa. Does not cross-react with ATF5.
- Cross-Reactivity
- Human, Rat
- Purification
- Protein G Purified
- Immunogen
- Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.
- Clone
- S360A-24
- Isotype
- IgG2a
- Top Product
- Discover our top product ATF4 Primary Antibody
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- Application Notes
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN1741066 was sufficient for detection of ATF4 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))
- Alternative Name
- ATF4 (ATF4 Products)
- Synonyms
- CREB-2 antibody, CREB2 antibody, TAXREB67 antibody, TXREB antibody, ATF4 antibody, atf-4 antibody, creb-2 antibody, creb2 antibody, taxreb67 antibody, txreb antibody, atf4 antibody, wu:fj01c08 antibody, wu:fk30b04 antibody, zgc:85951 antibody, sb:eu681 antibody, wu:fb08f07 antibody, zgc:171702 antibody, Atf-4 antibody, C/ATF antibody, ATF4-I antibody, atf4-a antibody, atf4-b antibody, atf4-ii antibody, activating transcription factor 4 antibody, activating transcription factor 4 L homeolog antibody, activating transcription factor 4a antibody, activating transcription factor 4b antibody, activating transcription factor 4 S homeolog antibody, ATF4 antibody, atf4 antibody, Atf4 antibody, atf4.L antibody, atf4a antibody, atf4b antibody, atf4.S antibody
- Background
- Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.
- Gene ID
- 468
- NCBI Accession
- NP_001666
- UniProt
- P18848
- Pathways
- Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response
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