GFAP antibody (AA 411-422) (PE)

Catalog No. ABIN1740979

Product Details anti-GFAP Antibody (PE)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Binding Specificity: AA 411-422

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~50 kDa. Does not cross-react with GFAP-R416W or other proteins (based on KO validation results).

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP, 100% identical in rat and mouse. >50% identity with other proteins (Vimentin, Desmin and Peripherin).

Clone: S206B-9

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1740979 was sufficient for detection of GFAP R416WT in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP (GFAP Products)

Synonyms: GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody

Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.

Gene ID: 2670

NCBI Accession: NP_001124491

UniProt: P14136