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CD14 antibody (AA 20-214)

Name: CD14 antibody (AA 20-214)
Brand: antibodies-online
SKU: ABIN1724926
Price: 511.5 USD
Availability: InStock

CD14 Reactivity: Human, Mouse FACS, WB, IHC, ELISA Host: Mouse Monoclonal 4B4F12 unconjugated

Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red). (CD14 antibody (AA 20-214))

FACS

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (CD14 antibody (AA 20-214))

ELISA

Western blot analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264. (CD14 antibody (AA 20-214))

Western blot analysis using CD14 mAb against human CD14 (AA: 20-214) recombinant protein. (CD14 antibody (AA 20-214))

Western blot analysis using CD14 mAb against HEK293 (1) and CD14 (AA: 20-214)-hIgGFc transfected HEK293 (2) cell lysate. (CD14 antibody (AA 20-214))

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD14 mouse mAb with DAB staining. (CD14 antibody (AA 20-214))

IHC

6 images

(2 references)

Catalog No. ABIN1724926

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Binding Specificity
9

6

5

5

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-214
Reactivity
221

37

30

25

22

18

16

15

14

13

13

10

9

9

8

8

8

5

2

2

2

1

1

1
Human, Mouse
Host
191

52

16

3

1
Mouse
Compatible Secondaries
Clonality
209

54
Monoclonal
Conjugate
107

30

22

17

13

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
196

78

55

49

45

44

20

13

13

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification

purified

Immunogen

Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. coli.

Clone

4B4F12

Isotype

IgG1

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anti-CD14 (CD14) antibody (Biotin)
CD14 Reactivity: Human, Non-Human Primate FACS, WB, ELISA, IP Host: Mouse Monoclonal MEM-18 Biotin

Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Matthews, Chung, Klasse, Moore, Sanders: "Potent induction of antibody-secreting B cells by human dermal-derived CD14+ dendritic cells triggered by dual TLR ligation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 12, pp. 5729-44, (2012) (PubMed).

Haghparast, Heidari Kharaji, Malvandi: "Down-regulation of CD14 transcripts in human glioblastoma cell line U87 MG." in: Iranian journal of immunology : IJI, Vol. 8, Issue 2, pp. 111-9, (2011) (PubMed).
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. , ,

Molecular Weight

40 kDa

Gene ID

929

HGNC

929

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades