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EGFR antibody (AA 693-893)

Name: EGFR antibody (AA 693-893)
Brand: antibodies-online
SKU: ABIN1724889
Price: 533.5 USD
Availability: InStock

EGFR Reactivity: Human WB, ELISA, FACS Host: Mouse Monoclonal 5E10D3 unconjugated

Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate. (EGFR antibody (AA 693-893))

Western blot analysis using EGFR mAb against human EGFR recombinant protein. (EGFR antibody (AA 693-893))

Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate. (EGFR antibody (AA 693-893))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (EGFR antibody (AA 693-893))

ELISA

Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red). (EGFR antibody (AA 693-893))

FACS

5 images

(2 references)

Catalog No. ABIN1724889

Datasheet as PDF

Target See all EGFR Antibodies

EGFR (Epidermal Growth Factor Receptor (EGFR))
Binding Specificity
40

39

31

30

30

27

26

26

21

21

19

16

16

16

15

15

15

15

11

11

10

10

9

9

8

8

7

6

5

5

5

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3
AA 693-893
Reactivity
744

293

283

26

26

24

19

14

8

3

2

2

1

1

1

1

1

1
Human
Host
580

165

14

13

5

1

1
Mouse
Compatible Secondaries
Clonality
534

240

2
Monoclonal
Conjugate
412

58

29

22

19

16

15

15

15

15

15

11

10

10

10

10

10

10

10

10

10

8

8

5

4

4

4

4

4

1

1

1

1

1

1
This EGFR antibody is un-conjugated
Application
536

245

150

144

129

102

101

95

82

49

37

35

34

18

11

7

6

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose

EGFR Antibody

Purification

Purified antibody

Immunogen

Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli.

Clone

5E10D3

Isotype

IgG2b

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Application Notes

ELISA: 1/10000
FCM: 1/200 - 1/400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Derer, Bauer, Lohse, Scheel, Berger, Kellner, Peipp, Valerius: "Impact of epidermal growth factor receptor (EGFR) cell surface expression levels on effector mechanisms of EGFR antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 11, pp. 5230-9, (2012) (PubMed).

Minder, Bayha, Becker-Pauly, Sterchi: "Meprin? transactivates the epidermal growth factor receptor (EGFR) via ligand shedding, thereby enhancing colorectal cancer cell proliferation and migration." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35201-11, (2012) (PubMed).
Target

EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name

EGFR (EGFR Products)

Background

Description: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Aliases: ERBB, HER1, mENA, ERBB1, PIG61

Molecular Weight

175kDa

Gene ID

1956

HGNC

1956

UniProt

P00533

Pathways

NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins