BRCA1 antibody (AA 229-335)

Catalog No. ABIN1724848

Product Details anti-BRCA1 Antibody

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Binding Specificity: AA 229-335

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purpose: BRCA1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human BRCA1 (AA: 229-335) expressed in E. Coli.

Clone: 6C6D2

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-BRCA1 antibody (ABIN1724848)

Muñoz, Laulier, Gunn, Cheng, Robbiani, Nussenzweig, Stark: "RING finger nuclear factor RNF168 is important for defects in homologous recombination caused by loss of the breast cancer susceptibility factor BRCA1." in: The Journal of biological chemistry, Vol. 287, Issue 48, pp. 40618-28, (2012) (PubMed).

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1 (BRCA1 Products)

Synonyms: BRCAI antibody, BRCC1 antibody, BROVCA1 antibody, IRIS antibody, PNCA4 antibody, PPP1R53 antibody, PSCP antibody, RNF53 antibody, iris antibody, pscp antibody, brcai antibody, brcc1 antibody, rnf53 antibody, brca1 antibody, BRCA1 antibody, LOC100224649 antibody, BRCA1, DNA repair associated antibody, breast cancer 1 antibody, breast cancer 1, early onset antibody, breast cancer 1 L homeolog antibody, BRCA1 antibody, brca1 antibody, LOC755086 antibody, Brca1 antibody, brca1.L antibody

Background:

Description: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Aliases: IRIS, PSCP, BRCAI, BRCC1, PNCA4, RNF53, BROVCA1, PPP1R53

Molecular Weight: 207.7kDa

Gene ID: 672

HGNC: 672

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus