PTK2B antibody (AA 815-997)

Catalog No. ABIN1724705

Product Details anti-PTK2B Antibody

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Binding Specificity: AA 815-997

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTK2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: PYK2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of PYK2 (aa815-997) expressed in E. Coli.

Clone: 5E2D5

Isotype: IgG2a

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-PTK2B antibody (ABIN1724705)

Dylla, Deyle, Theunissen, Padurean, Verfaillie: "Integrin engagement-induced inhibition of human myelopoiesis is mediated by proline-rich tyrosine kinase 2 gene products." in: Experimental hematology, Vol. 32, Issue 4, pp. 365-74, (2004) (PubMed).

Haglund, Ivankovic-Dikic, Shimokawa, Kruh, Dikic: "Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites." in: Journal of cell science, Vol. 117, Issue Pt 12, pp. 2557-68, (2004) (PubMed).

van der Horst, Weber, Ullrich: "Tyrosine phosphorylation of PYK2 mediates heregulin-induced glioma invasion: novel heregulin/HER3-stimulated signaling pathway in glioma." in: International journal of cancer. Journal international du cancer, Vol. 113, Issue 5, pp. 689-98, (2004) (PubMed).

Target Details for PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name: PYK2 (PTK2B Products)

Synonyms: CADTK antibody, CAKB antibody, FADK2 antibody, FAK2 antibody, PKB antibody, PTK antibody, PYK2 antibody, RAFTK antibody, CAKbeta antibody, E430023O05Rik antibody, Raftk antibody, Pyk2 antibody, fak1b antibody, ptk2.2 antibody, ptk2b antibody, si:ch1073-355e8.1 antibody, protein tyrosine kinase 2 beta antibody, PTK2 protein tyrosine kinase 2 beta antibody, protein tyrosine kinase 2aa antibody, PTK2B antibody, Ptk2b antibody, ptk2aa antibody

Background:

Description: PYK2: PTK2B protein tyrosine kinase 2 beta, also known as PTK2B, PKB, PTK, CAKB, FAK2, FRNK. Entrez Protein NP_004094. It is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.

Aliases: PKB, PTK, CAKB, FAK2, FRNK, PTK2B

Molecular Weight: 115.8kDa

Gene ID: 2185

NCBI Accession: NP_004094

HGNC: 2185

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling