DYKDDDDK Tag antibody

Catalog No. ABIN1724697

Product Details anti-DYKDDDDK Tag Antibody

Target: DYKDDDDK Tag

Reactivity: Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: Flag Antibody

Sequence: DYKDDDDK

Purification: Ascitic fluid

Immunogen: Synthetic peptide corresponding to aa(DYKDDDDK), conjugated to KLH.

Clone: 2F11G1

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/100-1/200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-DYKDDDDK Tag antibody (ABIN1724697)

Schuster, Wasserbauer, Einhauer, Ortner, Jungbauer, Hammerschmid, Werner: "Protein expression strategies for identification of novel target proteins." in: Journal of biomolecular screening, Vol. 5, Issue 2, pp. 89-97, (2000) (PubMed).

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: Flag (DYKDDDDK Tag Products)

Target Type: Tag

Background: The antibody is useful for the identification of proteins with Flag markers at free N-termini and C-termini by common immunological applications in yeast, animal, and E. coli cells.