HRP antibody

Catalog No. ABIN1724681

Product Details anti-HRP Antibody

Target: HRP (Horseradish Peroxidase (HRP))

Reactivity: Horseradish

Host: Mouse

Clonality: Monoclonal

Conjugate: This HRP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Immunogen: Purified recombinant fragment of HRP expressed in E. Coli.

Clone: 3A5C6

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution:
ELISA: 1/10000
WB: 1/500 - 1/2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C or at -20 °C for long term.

References for anti-HRP antibody (ABIN1724681)

Villegas: "Time course of angiogenesis in solid pineal autografts." in: Cell and tissue research, Vol. 315, Issue 3, pp. 349-59, (2004) (PubMed).

Metelitza, Karasyova, Grintsevich, Thorneley: "Peroxidase-catalyzed co-oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of substituted phenols and their polydisulfides." in: Journal of inorganic biochemistry, Vol. 98, Issue 1, pp. 1-9, (2003) (PubMed).

Target Details for HRP

Target: HRP (Horseradish Peroxidase (HRP))

Alternative Name: HRP (HRP Products)

Background: Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.