ERK2 antibody

Catalog No. ABIN1724654

Product Details anti-ERK2 Antibody

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: ERK2 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human ERK2 expressed in E. Coli.

Clone: 4C11

Isotype: IgG2a

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-ERK2 antibody (ABIN1724654)

Whitehurst, Robinson, Moore, Cobb: "The death effector domain protein PEA-15 prevents nuclear entry of ERK2 by inhibiting required interactions." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12840-7, (2004) (PubMed).

Hatano, Mori, Oh-hora, Kosugi, Fujikawa, Nakai, Niwa, Miyazaki, Hamaoka, Ogata: "Essential role for ERK2 mitogen-activated protein kinase in placental development." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 8, Issue 11, pp. 847-56, (2003) (PubMed).

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: ERK2 (MAPK1 Products)

Background:

Description: ERK2 (also designated extracellular-signal-related kinase 2 or mitogen-activated protein kinase 1), with 360-amino acid protein (about 40 kDa), belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.

Aliases: ERK, p38, p40, p41, ERT1, MAPK2, PRKM1, P42MAPK, p41mapk, MAPK1

Molecular Weight: 41kDa

Gene ID: 5594

HGNC: 5594

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins