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CD4 antibody (Cytosolic)

CD4 Reactivity: Human ELISA, IF Host: Mouse Monoclonal 8A5 unconjugated

Catalog No. ABIN1721491

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Binding Specificity
28

16

16

16

16

10

8

7

5

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cytosolic
Reactivity
339

202

112

48

24

20

19

18

18

12

10

10

10

10

10

9

9

9

8

5

4

3

1

1

1

1

1
Human
Host
344

150

112

3

3

1
Mouse
Compatible Secondaries
Clonality
468

139

3
Monoclonal
Conjugate
208

72

64

46

35

15

12

11

10

9

9

8

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD4 antibody is un-conjugated
Application
493

196

133

102

93

73

69

42

39

34

33

26

10

9

9

7

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1
ELISA, Immunofluorescence (IF)

Specificity

Reacts with the cytoplasmic domain of human CD4.

Purification

Purified

Immunogen

Peptide corresponding to the amino acid sequence RFLPADQMNVADPQPLASCS of human CD4.

Clone

8A5

Isotype

IgM

Top Product

Discover our top product CD4 Primary Antibody
anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 300-400) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

Application Notes

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA (10 - 20 μg/mL). Optimized for 25 μg cell lysate (Jurkat, H9, HeLa) on the solid phase. IF (5 - 10 μg/mL). Optimized for CEM cells adhered to glass slides with poly-L-lysine and fixed in paraformaldehyde.

Restrictions

For Research Use only
Buffer

PBS, pH 7.4

Handling Advice

Dilute in PBS or medium which is identical to that used in the assay system.

Storage

-20 °C

Storage Comment

Store at -20°C, stable for one year. Avoid repeated freeze thaw cycles.
Target

CD4

Alternative Name

CD4 (CD4 Products)

Synonyms

cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

Background

T-cell surface glycoprotein CD4 T-cell surface antigen T4/Leu-3 CD_antigen: CD4 Gene name : CD4

Gene ID

920

UniProt

P01730

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events