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CD4 antibody (Cytosolic)

CD4 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 10C12 unconjugated

Catalog No. ABIN1721490

Datasheet as PDF

Target See all CD4 Antibodies

CD4
Binding Specificity
28

16

16

16

16

10

8

7

5

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cytosolic
Reactivity
339

202

112

48

24

20

19

18

18

12

10

10

10

10

10

9

9

9

8

5

4

3

1

1

1

1

1
Human
Host
344

150

112

3

3

1
Mouse
Compatible Secondaries
Clonality
468

139

3
Monoclonal
Conjugate
208

72

64

46

35

15

12

11

10

9

9

8

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD4 antibody is un-conjugated
Application
493

195

133

102

93

73

69

42

39

34

33

26

10

9

9

7

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity

Reacts an epitope within amino acids 414-433 of the cytoplasmic tail region of human CD4.

Purification

Purified

Immunogen

Peptide corresponding to the amino acid sequence LSEKKTCQCPHRFQKTCSPI of human CD4.

Clone

10C12

Isotype

IgG1

Top Product

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anti-CD4 (CD4) (AA 385-457) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Pig, Cat, Cow, Rabbit, Sheep, Guinea Pig, Goat, Horse, Avian, Donkey, Hamster WB Host: Goat Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 Reactivity: Human FACS, WB, CyTOF Host: Mouse Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 300-400) antibody
CD4 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 Reactivity: Mouse FACS, IP, IHC (fro), ICC, Func Host: Rat Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 Reactivity: Human, Mouse, Rat FACS, WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 Reactivity: Human FACS Host: Mouse Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 Reactivity: Rat FACS Host: Mouse Monoclonal OX-35 PE

Application Notes

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
IF (5 - 10 μg/mL) Optimized for CEM cells adhered to glass slides with poly-L-lysine and fixed in paraformaldehyde. ELISA (10 - 20 μg/mL) Optimized for 25 μg cell lysate (Jurkat, H9, HeLa) on the solid phase. WB (5 - 10 μg/mL)

Restrictions

For Research Use only
Buffer

PBS, pH 7.4

Handling Advice

Dilute in PBS or medium which is identical to that used in the assay system.

Storage

-20 °C

Storage Comment

Store at -20°C, stable for one year. Avoid repeated freeze thaw cycles.
Target

CD4

Alternative Name

CD4 (CD4 Products)

Synonyms

cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

Background

T-cell surface glycoprotein CD4 T cell antigen T4 T cell differentiation antigen L3T4 T cell surface antigen T4/Leu 3 Gene name : CD4

Gene ID

920

UniProt

P01730

Pathways

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events