EPOR antibody

Catalog No. ABIN1720915

Product Details anti-EPOR Antibody

Target: EPOR (Erythropoietin Receptor (EPOR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPOR antibody is un-conjugated

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Specificity: Anti-human Epo-R

Characteristics: Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding the ECD of human Epo-R

Clone: VP-2E8

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Comment:

Synonyms: Erythropoietin receptor

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

References for anti-EPOR antibody (ABIN1720915)

Maxwell, Melendez-Rodríguez, Matchett, Aragones, Ben-Califa, Jaekel, Hengst, Lindner, Bernardini, Brockmeier, Fandrey, Grunert, Oster, Mittelman, El-Tanani, Thiersch, Schneider Gasser, Gassmann et al.: "Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. ..." in: British journal of haematology, Vol. 168, Issue 3, pp. 429-42, (2015) (PubMed).

Hedley, Allan, Xenocostas: "The role of erythropoietin and erythropoiesis-stimulating agents in tumor progression." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 20, pp. 6373-80, (2011) (PubMed).

Target Details for EPOR

Target: EPOR (Erythropoietin Receptor (EPOR))

Alternative Name: EPOR

Background: Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo). Erythropoietin is the primary regulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the Epo receptor. The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance.

UniProt: P19235

Pathways: JAK-STAT Signaling