MAPK6 antibody (AA 21-120)

Catalog No. ABIN1714846

Product Details anti-MAPK6 Antibody

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Binding Specificity: AA 21-120

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MAPK6/ERK3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Alternative Name: MAPK6/ERK3 (MAPK6 Products)

Background:

Synonyms: ERK-3, ERK3, ERK 3, ERK-3, Extracellular signal regulated kinase 3, Extracellular signal regulated kinase p97, Extracellular signal-regulated kinase 3, MAP kinase 6, MAP kinase isoform p97, MAPK 6, MAPK6, Mitogen activated protein kinase 6, Mitogen-activated protein kinase 6, MK06_HUMAN, p97 MAPK, p97-MAPK, PRKM6, Protein kinase mitogen activated 5, Protein kinase mitogen activated 6.

Background: Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl-2 and caspase-3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK 3 gene maps to chromosome 15q21.2 and encodes a 721 amino acid protein.

Gene ID: 5597

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C