GEM antibody (AA 201-296)

Catalog No. ABIN1714797

Product Details anti-GEM Antibody

Target: GEM (GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))

Binding Specificity: AA 201-296

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GEM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GEM

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GEM

Target: GEM (GTP Binding Protein Overexpressed in Skeletal Muscle (GEM))

Alternative Name: GEM (GEM Products)

Synonyms: KIR antibody, GEM antibody, DKFZp470D0612 antibody, LOC100221129 antibody, kir antibody, xgem antibody, zgc:153003 antibody, Gem antibody, AV020497 antibody, GTP binding protein overexpressed in skeletal muscle antibody, geminin, DNA replication inhibitor antibody, GTP binding protein (gene overexpressed in skeletal muscle) antibody, GEM antibody, gem antibody, Gem antibody, GMNN antibody

Background:

Synonyms: GTP binding mitogen induced T cell protein, GTP binding protein expressed in mitogen stimulated T cells, GTP binding protein GEM, GTP binding protein overexpressed in skeletal muscle, Kinase inducible Ras like protein, KIR, MGC26294, RAS like protein KIR, GEM_HUMAN.

Background: Gem belongs to the Rad/Gem/Kir (RGK) subfamily of Ras-related GTPases, which lack typical C-terminal amino acid motifs for isoprenylation. Rad and Gem bind calmodulin in a Ca2+-dependent manner via this C-terminal extension, involving residues 278297 in human Rad. High intracellular Gem levels, which interact with intact microtubules and microfilaments, promote profound changes in cell morphology. Ectopic Gem expression is sufficient to stimulate cell flattening and neurite extension in N1E-115 and SH-SY5Y neuroblastoma cells, suggesting a role for Gem in cytoskeletal rearrangement and/or morphological differentiation of neurons. Gem was also observed in developing trigeminal nerve ganglia in 12.5 day mouse embryos, demonstrating that Gem expression is a property of normal ganglionic development. The interaction of Gem with beta-subunits regulates Ca2+ channel expression at the cell surface. The human Gem gene maps to chromosome ,8q22.1.

Gene ID: 2669