AGO2 antibody (AA 431-530)

Catalog No. ABIN1714738

Product Details anti-AGO2 Antibody

Target: AGO2 (Argonaute 2 (AGO2))

Binding Specificity: AA 431-530

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human eIF2C2/Ago2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for AGO2

Target: AGO2 (Argonaute 2 (AGO2))

Alternative Name: e2C2/Ago2 (AGO2 Products)

Synonyms: EIF2C2 antibody, T19E23.7 antibody, T19E23_7 antibody, argonaute 2 antibody, Q10 antibody, Eif2c2 antibody, Argonaute2 antibody, eif2c1 antibody, eif2c2 antibody, 1110029L17Rik antibody, 2310051F07Rik antibody, AI225898 antibody, AL022874 antibody, AW546247 antibody, ENSMUSG00000072493 antibody, Gerp95 antibody, Gm10365 antibody, mKIAA4215 antibody, AGO2 antibody, AG02 antibody, AGO 2 antibody, Ago-2 antibody, Ago2 antibody, CG13452 antibody, CG7439 antibody, Dm Ago2 antibody, Dmel\\CG7439 antibody, ago antibody, ago-2 antibody, ago2 antibody, dAGO2 antibody, dAgo2 antibody, eIF2C2 antibody, argonaute 2, RISC catalytic component antibody, Argonaute family protein antibody, argonaute 2, RISC catalytic component L homeolog antibody, argonaute RISC catalytic subunit 2 antibody, argonaute RISC catalytic component 2 antibody, Argonaute 2 antibody, AGO2 antibody, Ago2 antibody, ago2.L antibody, ago2 antibody

Background:

Synonyms: Ago 2, AGO2_HUMAN, Argonaute 2, Argonaute2, dAgo2, e 2C 2, e-2C 2, e2C 2, E2c2, Eukaryotic translation initiation factor 2C 2, hAgo2, MGC3183, PAZ Piwi domain protein, PPD, Protein argonaute-2, Protein slicer, Q10, Slicer protein.

Background: Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

Gene ID: 27161

UniProt: Q9UKV8

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization