BOLL antibody (AA 1-100)

Catalog No. ABIN1714670

Product Details anti-BOLL Antibody

Target: BOLL (Bol, Boule-Like (BOLL))

Binding Specificity: AA 1-100

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BOLL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Cross-Reactivity: Sheep

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Monkey

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BOULE

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for BOLL

Target: BOLL (Bol, Boule-Like (BOLL))

Alternative Name: BOULE (BOLL Products)

Synonyms: BOULE antibody, 4930554P13Rik antibody, 4930597B14Rik antibody, RGD1559527 antibody, boule homolog, RNA binding protein antibody, bol, boule-like (Drosophila) antibody, BOLL antibody, Boll antibody

Background:

Synonyms: Bol Drosophila boule homolog like, Bol, Bol boule like, Bol, boule like Drosophila, bol, boule-like Drosophila, Boll, BOLL_HUMAN, BOULE, Boule like, BOULE, Drosophila, homolog of, Protein boule like, Protein boule-like, Putative uncharacterized protein BOLL.

Background: Spermatogenesis represents the intricate developmental process of mitotic and meiotic cell divisions that ultimately leads to the production of haploid spermatozoa. BOULE, a member of the human deleted in azoospermia (DAZ) family, functions as a key conserved switch that regulates the progression of germ cells through meiosis in man. BOULE is an RNA-binding protein that regulates the expression of twine, a Cdc25 phosphatase, which promotes progression through meiosis. BOULE is expressed not only in the testis, but also in the nervous system, where it may play a role in neural communication. Mutations in the BOULE gene are be associated with male infertility, and the relative proportions of the three BOULE isoforms (B1, B2 and B3) may function as predictive markers for meiotic efficiency.

Gene ID: 66037