GUCA1B antibody (AA 21-120)

Catalog No. ABIN1714375

Product Details anti-GUCA1B Antibody

Target: GUCA1B (Guanylate Cyclase Activator 1B (Retina) (GUCA1B))

Binding Specificity: AA 21-120

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GUCA1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc))

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GCAP2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GUCA1B

Target: GUCA1B (Guanylate Cyclase Activator 1B (Retina) (GUCA1B))

Alternative Name: GCAP2 (GUCA1B Products)

Synonyms: GUCA1B antibody, BC018258 antibody, GCAP2 antibody, GUCA2 antibody, RP48 antibody, guanylate cyclase activator 1B antibody, guanylate cyclase activator 1B (retina) antibody, GUCA1B antibody, guca1b antibody, Guca1b antibody

Background:

Synonyms: GCAP 2, Guanylate Cyclase Activating Protein Photoreceptor 2, Guanylate Cyclase Activator 1B, Guanylin 2, Guanylyl cyclase-activating protein 2, GUC1B_HUMAN, GUCA 2, GUCA1B.

Background: The intracellular stimulation of guanylate cyclase (GC) by calcium, a key event in the recovery of the dark state of rod photoreceptors after exposure to light, is mediated by guanylate cyclase-activating protein (GCAP1). GCAPs are calcium-binding proteins belonging to the calmodulin superfamily. GCAP1 is a calcium-binding protein that stimulates synthesis of cGMP in photoreceptors. GCAP1 is present in rod and cone photoreceptor outer segments where phototransduction occurs. In contrast to other calcium-binding proteins from the calmodulin superfamily, the calcium-free form of GCAP1 stimulates the effector enzyme. By molecular cloning of human and mouse GCAP cDNA, the known mammalian GCAPs are found to be more than 90 % similar, consisting of 201 to 205 amino acids and containing three identically conserved calcium-binding sites. A related protein, GCAP2, is detectable only in the retina and results from a gene duplication event. The genes which encode GCAP1 and GCAP2 map to human chromosome 6p21.1.

Gene ID: 2979

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction