ARIH2 antibody (AA 41-140)

Catalog No. ABIN1714290

Product Details anti-ARIH2 Antibody

Target: ARIH2 (protein ariadne-2 homolog (ARIH2))

Binding Specificity: AA 41-140

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARIH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ARIH2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ARIH2

Target: ARIH2 (protein ariadne-2 homolog (ARIH2))

Alternative Name: ARIH2 (ARIH2 Products)

Synonyms: ARI2 antibody, TRIAD1 antibody, AI843547 antibody, UIP48 antibody, wu:fa09c12 antibody, zgc:64093 antibody, ariadne RBR E3 ubiquitin protein ligase 2 antibody, ariadne RBR E3 ubiquitin protein ligase 2 L homeolog antibody, ariadne homolog 2 (Drosophila) antibody, ARIH2 antibody, Arih2 antibody, arih2.L antibody, arih2 antibody

Background:

Synonyms: all trans retinoic acid inducible RING finger, Ari 2, Ari-2, ARI2, ARI2_HUMAN, Ariadne 2, Ariadne 2 protein homolog, ariadne homolog 2 Drosophila, ariadne homolog 2, ARIH 2, Arih2, E3 ubiquitin protein ligase ARIH2, E3 ubiquitin-protein ligase ARIH2, HT005, protein ariadne 2 homolog, Protein ariadne-2 homolog, Triad 1, TRIAD1, Triad1 protein, UIP48.

Background: TRIAD1, also known as ARIH2 (ariadne homolog 2) or ARI2, is a 493 amino acid protein that contains one IBR-type zinc finger and two RING-type zinc fingers and belongs to the ariadne subfamily of RBR proteins. Localized to the nucleus, TRIAD1 interacts with UBE2L3 and is thought to act as an E3 ubiquitin-protein ligase, functioning to accept ubiquitin from E2 ubiquitin-conjugating enzymes and transfer the acquired ubiquitin residue to target substrates. TRIAD1 is subject to post-translational DNA damage-dependent phosphorylation, probably by ATM or ATR. The gene encoding TRIAD1 maps to human chromosome 3, which houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci.

Gene ID: 10425