PBX3 antibody (AA 65-130)

Catalog No. ABIN1714219

Product Details anti-PBX3 Antibody

Target: PBX3 (Pre-B-Cell Leukemia Transcription Factor 3 (PBX3))

Binding Specificity: AA 65-130

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PBX3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PBX3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-PBX3 antibody (ABIN1714219)

Ma, Zhang, Mou, Liu, Ru, Li: "High level of homeobox A9 and PBX homeobox 3 expression in gastric cancer correlates with poor prognosis." in: Oncology letters, Vol. 14, Issue 5, pp. 5883-5889, (2017) (PubMed).

Target Details for PBX3

Target: PBX3 (Pre-B-Cell Leukemia Transcription Factor 3 (PBX3))

Alternative Name: PBX3 (PBX3 Products)

Synonyms: Pbx3b antibody, pre B cell leukemia homeobox 3 antibody, PBX homeobox 3 antibody, Pbx3 antibody, PBX3 antibody

Background:

Synonyms: Homeobox protein PBX3, MGC134053, OTTHUMP00000064213, Pbx 3, Pbx3, PBX3_HUMAN, Pre B cell leukemia homeobox 3, Pre B cell leukemia transcription factor 3, Pre-B-cell leukemia transcription factor 3, RP11 336P12.1.

Background: Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Human pre-B cell acute leukemias are frequently associated with a t(1,19)(q23,p13.3) chromosomal rearrangement, which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Pbx 2 and Pbx 3 share 92 % and 94 % respective identities with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase.

Gene ID: 5090