EPRS antibody (AA 651-750)

Catalog No. ABIN1714190

Product Details anti-EPRS Antibody

Target: EPRS (Glutamyl-Prolyl-tRNA Synthetase (EPRS))

Binding Specificity: AA 651-750

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPRS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Glutamyl Prolyl tRNA syntase/ProRS

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for EPRS

Target: EPRS (Glutamyl-Prolyl-tRNA Synthetase (EPRS))

Alternative Name: Glutamyl Prolyl tRNA synthetase/ProRS (EPRS Products)

Synonyms: EARS antibody, GLUPRORS antibody, PARS antibody, QARS antibody, QPRS antibody, wu:fb38d08 antibody, wu:ft34d10 antibody, 2410081F06Rik antibody, 3010002K18Rik antibody, C79379 antibody, Qprs antibody, glutamyl-prolyl-tRNA synthetase antibody, glutamyl-prolyl-tRNA synthetase S homeolog antibody, EPRS antibody, eprs antibody, eprs.S antibody, Eprs antibody

Background:

Synonyms: EARS, PARS, QARS, QPRS, PIG32, GLUPRORS, Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase, Cell proliferation-inducing gene 32 protein, Glutamatyl-prolyl-tRNA synthetase, EPRS, GLNS

Background: Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the multisynthetase complex and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Gene ID: 2058

UniProt: P07814