DMAP1 antibody (AA 31-130)

Catalog No. ABIN1714047

Product Details anti-DMAP1 Antibody

Target: DMAP1 (DNA Methyltransferase 1-Associated Protein 1 (DMAP1))

Binding Specificity: AA 31-130

Reactivity: Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DMAP1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Xenopus laevis

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DMAP1

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DMAP1

Target: DMAP1 (DNA Methyltransferase 1-Associated Protein 1 (DMAP1))

Alternative Name: DMAP1 (DMAP1 Products)

Background:

Synonyms: DKFZp686L09142, DMAP 1, DNA methyltransferase 1-associated protein 1, DNMAP 1, DNMAP1, DNMT1-associated protein 1, DNMT1-associated protein 1, DNMTAP 1, DNMTAP1, EAF 2, EAF2, FLJ11543, KIAA1425, MAT 1-mediated transcriptional repressor, MGC55593, SWC 4, SWC4, zgc:55593.

Background: Methylation of DNA contributes to the regulation of gene transcription in eukaryotic systems. DNA methylation is predominantly found on cytosine residues that are present in dinucleotide motifs consisting of a 5' cytosine followed by a guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferases (DNMTs), which results in transcriptional repression of the methylated gene. DNA methyltransferase 1-associating protein (Dmap1) binds to methyl-CpG rich domains and mediate the transcriptional inhibition associated with DNA methylation. Dmap1 interacts with Daxx to enhanced Daxx-mediated repression of glucocorticoid receptor transcriptional activity. Daxx also protects Dmap1 from protein degradation in vivo.

Gene ID: 55929