CSTF3 antibody (AA 151-250)
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- Target See all CSTF3 Antibodies
- CSTF3 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3))
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Binding Specificity
- AA 151-250
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CSTF3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Cross-Reactivity
- Mouse
- Predicted Reactivity
- Human,Rat,Dog,Cow,Chicken,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human CSTF3/CSTF77
- Isotype
- IgG
- Top Product
- Discover our top product CSTF3 Primary Antibody
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- CSTF3 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3))
- Alternative Name
- CSTF3/CSTF77 (CSTF3 Products)
- Synonyms
- cstf3 antibody, MGC76035 antibody, CSTF3 antibody, wu:fa99c02 antibody, wu:fb18b09 antibody, zgc:56028 antibody, 4732468G05Rik antibody, C79532 antibody, CstF-77 antibody, cstf3a antibody, CSTF-77 antibody, cleavage stimulation factor, 3' pre-RNA, subunit 3 antibody, cleavage stimulation factor subunit 3 antibody, cstf3 antibody, CSTF3 antibody, LOC100544392 antibody, LOC100641283 antibody, Cstf3 antibody, cstf3.S antibody
- Background
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Synonyms: CF 1 77 kDa subunit, Cleavage stimulation factor 77 kDa subunit, Cleavage stimulation factor subunit 3, Cleavage stimulation factor subunit 3 isoform 1, CSTF 3, CSTF 77, CSTF 77 kDa subunit, CSTF77, MGC117398, MGC43001, MGC75122, CSTF3_HUMAN.
Background: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation.
- Gene ID
- 1479
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