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NGEF antibody (pTyr179)

NGEF Reactivity: Human, Mouse, Rat WB, ELISA, IF (p), IF (cc), IHC (fro), IHC (p), ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1713981
  • Target See all NGEF Antibodies
    NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))
    Binding Specificity
    • 15
    • 14
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr179
    Reactivity
    • 40
    • 40
    • 33
    • 3
    • 3
    • 3
    • 3
    Human, Mouse, Rat
    Host
    • 47
    • 1
    Rabbit
    Clonality
    • 47
    • 1
    Polyclonal
    Conjugate
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NGEF antibody is un-conjugated
    Application
    • 38
    • 25
    • 25
    • 10
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Chicken
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human Ephexin-1 around the phosphorylation site of Tyr179
    Isotype
    IgG
    Top Product
    Discover our top product NGEF Primary Antibody
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    ICC 1:100-500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))
    Alternative Name
    Ephexin-1 (NGEF Products)
    Synonyms
    Ephexin-1 antibody, ARHGEF27 antibody, EPHEXIN antibody, Tims2 antibody, ephexin antibody, Besh3 antibody, ephexin-1 antibody, neuronal guanine nucleotide exchange factor antibody, NGEF antibody, Ngef antibody
    Background

    Synonyms: Ephexin-1 phospho Y179, p-Ephexin-1 phospho Y179, ARHGEF27, Eph-interacting exchange protein, EPHEXIN, Ephexin 1, Neuronal guanine nucleotide exchange factor, Ngef, NGEF_HUMAN.

    Background: EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species.

    Gene ID
    25791
    Pathways
    Neurotrophin Signaling Pathway
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