TAZ antibody (AA 1-100)

Catalog No. ABIN1713913

Product Details anti-TAZ Antibody

Target: TAZ (Tafazzin (TAZ))

Binding Specificity: AA 1-100

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAZ antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TAZ

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TAZ antibody (ABIN1713913)

Ukita, Yamaguchi, Ohata, Tamura: "Sclerostin Enhances Adipocyte Differentiation in 3T3-L1 Cells." in: Journal of cellular biochemistry, Vol. 117, Issue 6, pp. 1419-28, (2016) (PubMed).

Sun, Chen, Shi, Qi, Zhao, Zhang: "Prognostic impact of TAZ and ?-catenin expression in adenocarcinoma of the esophagogastric junction." in: Diagnostic pathology, Vol. 9, pp. 125, (2014) (PubMed).

Target Details for TAZ

Target: TAZ (Tafazzin (TAZ))

Alternative Name: TAZ (TAZ Products)

Background:

Synonyms: TAZ, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, WWTR1

Background: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.

Gene ID: 25937

UniProt: Q9GZV5