Actin antibody (AA 165-260)

Catalog No. ABIN1713700

Product Details anti-Actin Antibody

Target: Actin

Binding Specificity: AA 165-260

Reactivity: Human, Rat, Mouse, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: Alpha, Beta and Gamma Actins

Cross-Reactivity: Dog, Human, Mouse, Rabbit, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human alpha smooth muscle Actin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Actin antibody (ABIN1713700)

Wang, Du, Niu, Fu, Wang, Zhang, Zhao, Sun, Nan: "MiR-130a-3p attenuates activation and induces apoptosis of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis by directly targeting TGFBR1 and TGFBR2." in: Cell death & disease, Vol. 8, Issue 5, pp. e2792, (2018) (PubMed).

Park, Kim: "Cigarette smoke-induced EGFR activation promotes epithelial mesenchymal migration of human retinal pigment epithelial cells through regulation of the FAK-mediated Syk/Src pathway." in: Molecular medicine reports, Vol. 17, Issue 3, pp. 3563-3574, (2018) (PubMed).

Shen, Wu, Wang, Zhao, Zhang, Li, Zhao: "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." in: International immunopharmacology, Vol. 46, pp. 16-22, (2017) (PubMed).

Kim, Ko, Park, Hur, Kim, Yang: "Vandetanib and ADAM inhibitors synergistically attenuate the pathological migration of EBV-infected retinal pigment epithelial cells by regulating the VEGF-mediated MAPK pathway." in: Experimental and therapeutic medicine, Vol. 13, Issue 4, pp. 1415-1425, (2017) (PubMed).

Pang, Bai, Wu, Chen, Zhang, Tang: "Urotensin II Induces ER Stress and EMT and Increase Extracellular Matrix Production in Renal Tubular Epithelial Cell in Early Diabetic Mice." in: Kidney & blood pressure research, Vol. 41, Issue 4, pp. 434-49, (2016) (PubMed).

Gan, Jing, Zhu, Qin, Teng, Guo: "Preventive effects of ¹²⁵I seeds on benign restenosis following esophageal stent implantation in a dog model." in: Molecular medicine reports, Vol. 11, Issue 5, pp. 3382-90, (2016) (PubMed).

Li, Li, Xu, Zhao, Deng, Mo, Li, Qi, Li, Gao: "Total extract of Yupingfeng attenuates bleomycin-induced pulmonary fibrosis in rats." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 22, Issue 1, pp. 111-9, (2015) (PubMed).

Qiao, Wang, Wang, Zhao, Zhang, Han, Peng: "Intermedin is upregulated and attenuates renal fibrosis by inhibition of oxidative stress in rats with unilateral ureteral obstruction." in: Nephrology (Carlton, Vic.), (2015) (PubMed).

Yan, Wu, Li, Meng, Dai, Zheng, Fu: "Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-?-Treated Rat Aortic Smooth Muscle Cells." in: Cardiovascular toxicology, (2015) (PubMed).

Xu, Li, Zhao, Qi, Li, Gao, Fei: "Total polysaccharide of Yupingfeng protects against bleomycin-induced pulmonary fibrosis via inhibiting transforming growth factor-?1-mediated type I collagen abnormal deposition in rats." in: The Journal of pharmacy and pharmacology, (2014) (PubMed).

Gao, Zhang, Ding, Chen, Hu, Jiang, Li, Chen, Wang, Ye, Zhu: "Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells." in: Cell transplantation, Vol. 22, Issue 10, pp. 1883-900, (2014) (PubMed).

Target Details for Actin

Target: Actin

Abstract: Actin Products

Background:

Synonyms: Actin, alpha skeletal muscle, ACTA1, Alpha-actin-1, Actin, alpha cardiac muscle 1, ACTC1, Alpha-cardiac actin, Actin, aortic smooth muscle, ACTA2, Alpha-actin-2, Actin, gamma-enteric smooth muscle, ACTG2, Actin, cytoplasmic 2, ACTG1, Actin, cytoplasmic 1, Gamma-actin, ACTB, Beta-actin,

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Gene ID: 70

UniProt: P68032