LIMS2 antibody (AA 251-341)

Catalog No. ABIN1713667

Product Details anti-LIMS2 Antibody

Target: LIMS2 (LIM and Senescent Cell Antigen-Like Domains 2 (LIMS2))

Binding Specificity: AA 251-341

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMS2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LIMS2

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for LIMS2

Target: LIMS2 (LIM and Senescent Cell Antigen-Like Domains 2 (LIMS2))

Alternative Name: LIMS2 (LIMS2 Products)

Synonyms: PINCH2 antibody, PINCH-2 antibody, LIMS1 antibody, LIM and senescent cell antigen like domains 2 antibody, LIM zinc finger domain containing 2 antibody, Lims2 antibody, LIMS2 antibody

Background:

Synonyms: FLJ10044, ILK binding protein, LIM and senescent cell antigen like domains 2, LIM and senescent cell antigen-like-containing domain protein 2, LIM-like protein 2, LIMS2, LIMS2_HUMAN, Particularly interesting new Cys-His protein 2, PINCH 2, PINCH-2, PINCH2.

Background: Pinch is a focal adhesion protein that is a component of the ILK-PINCH complex. This complex is a major part of the growth factor and integrin signaling pathway. Pinch is involved in cell differentiation, proliferation and survival by acting as an effector of integrin and growth factor signaling. It is a cytoplasmic protein expressed in most tissues and consists of five LIM domains, a nuclear localization signal and a nuclear export signal. The PINCH-1/ILK complex is regulated by another member of the Pinch family, PINCH-2, which also forms a complex with ILK.

Pathways: Cell-Cell Junction Organization