Girdin antibody (pTyr1765)

Catalog No. ABIN1713601

Product Details anti-Girdin Antibody

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Binding Specificity: pTyr1765

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Girdin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human APE around the phosphorylation site of Tyr1765

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Girdin

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Alternative Name: Girdin (CCDC88A Products)

Background:

Synonyms: Girdin phospho Y1765, p-Girdin phospho Y1765, Akt phosphorylation enhancer, APE, Coiled coil domain containing protein 88A, G alpha interacting vesicle associated protein, Girders of actin filament, GIV, HkRP1, Hook related protein 1, AKT iphosphorylation enhancer, Akt phosphorylation enhancer, Ccdc88a, GIV, GRDN, GRDN_HUMAN, HkRP1, Hook related protein 1, Hook-related protein 1, KIAA1212.

Background: Girdin is a 1,871 amino acid protein that, via interactions with phosphoinositides, localizes to the cell membrane and to the cytosol. Expressed ubiquitously, girdin functions to enhance the activity of PI 3-kinase-dependent phosphorylation of proteins, such as Akt1, thereby inducing the phosphorylation of downstream protein targets and, ultimately, regulating DNA replication and cellular proliferation. Additionally, girdin is crucial for cell migration and is responsible for both maintaining the structural integrity of the Actin cytoskeleton and for regulating the formation of Actin stress fibers. Girdin exists as a homodimer that can itself be phosphorylatedan event that delocalizes girdin from the cell membrane, thus allowing it to participate in cell migration events. Two isoforms of girdin exist due to alternative splicing events.

Gene ID: 55704