Ly6c/CD59 antibody (AA 26-102)

Catalog No. ABIN1713557

Product Details anti-Ly6c/CD59 Antibody

Target: Ly6c/CD59 (LY6C) (Lymphocyte Antigen 6 Complex, Locus C (LY6C))

Binding Specificity: AA 26-102

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ly6c/CD59 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ly6c

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Ly6c/CD59 antibody (ABIN1713557)

Nashine, Chwa, Kazemian, Thaker, Lu, Nesburn, Kuppermann, Kenney: "Differential Expression of Complement Markers in Normal and AMD Transmitochondrial Cybrids." in: PLoS ONE, Vol. 11, Issue 8, pp. e0159828, (2017) (PubMed).

Target Details for Ly6c/CD59

Target: Ly6c/CD59 (LY6C) (Lymphocyte Antigen 6 Complex, Locus C (LY6C))

Alternative Name: Ly6c/CD59 (LY6C Products)

Background:

Synonyms: Ly 6c, Ly6c protein, Ly-6C, Ly6c1, Ly6c2, Lymphocyte antigen 6 complex, Lymphocyte antigen 6 complex locus C, Lymphocyte antigen 6C, Lymphocyte antigen 6C1, Lymphocyte antigen 6C2, Lymphocyte antigen Ly 6C, CD59_HUMAN.

Background: The gene encoding the mouse alloantigen, Ly-6C, maps to chromosome 15 and encodes a 131 amino acid protein that belongs to the Ly-6 family of glycosyl-phosphatidylinositol (GPI)-linked proteins. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. Murine Ly-6 Molecules have unique patterns of tissue expression during hematopoiesis from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6C is predominantly expressed on murine peripheral CD8 T cells. Ly-6C is involved in endothelial adhesion, the killing of target cells by CTLs, inducing TCR-mediated activation of IL-2 and IFN-?production in CD8 T cells and the homing of CD8 T cells. In addition, Ly-6C may act as a signaling molecule of LFA-1 activation.