PRAM1 antibody (AA 551-650) (HRP)

Catalog No. ABIN1711399

Product Details anti-PRAM1 Antibody (HRP)

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Binding Specificity: AA 551-650

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAM1 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PRAM1

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRAM1

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Alternative Name: PRAM1 (PRAM1 Products)

Synonyms: PRAM1 antibody, AY665714 antibody, PML-RAR antibody, PRAM-1 antibody, RGD1564330 antibody, PML-RARA regulated adaptor molecule 1 antibody, PML-RAR alpha-regulated adaptor molecule 1 antibody, PRAM1 antibody, Pram1 antibody

Background:

Synonyms: PML RAR, PML RARA regulated adaptor molecule 1, PML RARA target gene encoding an Adaptor Molecule 1, PML-RARA-regulated adapter molecule 1, PRAM 1, PRAM, PRAM-1, PRAM_HUMAN, PRAM1.

Background: Complete remission of acute promyelocytic leukemia can be achieved by treating patients with retinoic acid, and PML-RAR-a (promyelocytic leukemia-retinoic acid receptor alpha fusion protein) plays a major role in mediating retinoic acid effects in leukemia cells. The retinoic acid-induced gene, PRAM-1 (PML-RAR-a target gene encoding an adaptor molecule 1) encodes an adaptor protein which is expressed and modulated during normal human myelopoiesis. PRAM-1 expression is hindered by expression of PML-RAR-a. The 718 amino acid PRAM-1 protein contains eight N-terminal proline-rich repeats and several proline residues that are clustered as type I or type II SH3 recognition motifs. PRAM-1 demonstrates expression in hematopoietic tissues and lung.

Pathways: Regulation of Leukocyte Mediated Immunity