POLM antibody (AA 261-360) (HRP)

Catalog No. ABIN1711280

Product Details anti-POLM Antibody (HRP)

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Binding Specificity: AA 261-360

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLM antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA polymerase mu

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for POLM

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Alternative Name: DNA polymerase mu (POLM Products)

Synonyms: polmu antibody, zgc:56122 antibody, POLM antibody, Pol Mu antibody, Tdt-N antibody, AI462166 antibody, B230309I03Rik antibody, polymerase (DNA directed), mu antibody, DNA polymerase mu antibody, polm antibody, POLM antibody, CC1G_09351 antibody, VDBG_09335 antibody, Polm antibody

Background:

Synonyms: DNA Polymerase Mu, DNA-directed DNA polymerase mu, DNA-directed DNA/RNA polymerase mu, DPOLM_HUMAN, FLJ35482, Pol iota, Pol Mu, Polm, POLM protein, Polymerase DNA directed mu, Polymerase DNA directed mu, Tdt N, Tdt-N, TdtN, Terminal transferase.

Background: DNA polymerase mu shares a number of characteristics with DNA polymerase Beta as well as with terminal deoxynucleotideyltransferase. Pol mu purportedly plays a role in microhomology mediated joining and the repair of double-stranded breaks. However, unlike other DNA polymerases, which show substrate specificity for deoxynucleotides, DNA Pol mu incorporates both deoxynucleotides and ribonucleotides in a template- directed manner. This unusual capability implies a novel role for this polymerase in DNA repair.

Gene ID: 27434

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response